How does radiation/chemotherapy affect tumor cancer cell-line growth?
Does low dose(smaller than 50 rads? - Stanziale et al.) help or inhibit growth? What dose provides the most optimal growth condition for tumor cell lines? (2)
Does radiation/chemotherapy confer a selective advantage to a particular cell line? What dose singles out a cell line from the rest? (3)
Does a large single dose(a large disturbance) more effectively inhibit growth or do smaller doses(small disturbances) that equal the large dose in total amount provide more effective inhibition? (4)
How's the recovery of the tumor community after a large single dose? After a number of smaller doses? (5)
How do cancer cell-lines interact with each other in an experimental laboratory setting?(1)
what happens when two to three species are grown together in vitro? Do the associations exhibit synergistic, additive or antagonistic effects on different cells' growth?
Does each species exhibit greater growth(volume) when grown separately or grown together? (ecology p188)
Cell lines A, B and C are propagated separately. Volume of the growth is measured after a week(5 plates per species). A & B grown together(total of 5 plates), B&C grown together(total of 5 plates), A&C grown together(total of 5 plates), A&B&C are grown together(total of 5 plates). Volumes of cell lines are measured after a week's time.
Separete A, B and C plates are prepared(15 plates each). Each cell line receives 5 different levels of radiation doses. Their volumes are measured after a week and compared with (1)'s each cell line growth.
A&B&C plates(15 plates are prepared) receive 5 different levels of radiation doses. Their volumes are measured after a week and compared with (1)'s A&B&C growth pattern.
Individual cell lines(10 plates each) are divided into two groups where one group(5 plates per cell line) receive a large single dose while another group receives a number of smaller doses. Their volumes are measured after a week's time, or after the treatment.
(4)'s viable cells are inoculated into new media for each cell line(5 plates per cell line). Their growth rate(recovery) is measured by measuring cell volume at respective time interval(5 plates for 5 intervals).
